The Effect of Estrogen and Progesterone on Proliferation of Ovarian Cancer Cell Line in Vitro

Objective To investigate the effect of 17-β estrogen and progesterone on cell proliferation of human ovarian cancer in vitro.Methods The proliferation capacity of the ovarian carcinoma cell line HO_ 8910 in the culture medium with 17-β estrogen and progesterone was evaluated by the micoculture tetrazolium assay (MTT).The expression of the estrogen receptor,progesterone receptor and androgen receptor of the cell line was examined by the immunohistchemical staining. The cell apoptosis was detected by DNA agarose gel electrophoresis and deoxynucleotidyl transferase-mediated duTP nick end labeling (TUNEL).Results After 48 and 72 hours,the growth of HO_ 8910 cell line was inhibited by the mixture of 17-β estrogen and progesterone (1×10 -10 mol/L、1×10 -6 ～1×10 -5 mol/L). The mixture in concentration of 1×10 -5 mol/L in 21 days inhibited the proliferation of cell too. There were no singnificant difference in estrogen receptor,progesterone receptor and androgen receptor of HO_ 8910 treaded with 17-β estrogen and progesterone. The result of no evidence of apoptosis in cell line treated with 17-β estrogen and progesterone.Conclusion The mixture of estrogen and progesterone could inhibit the proliferation of ovarian cell.But the expression of steroid hormone receptor could not be changed,and apoptosis in ovarian cell line could not be introduced.