The Effect of Estrogen on Proliferation of Ovarian Cancer Cell Line in Vitro

Objective To investigate the effect of 17-βestrogen on cell proliferation of human ovarian cancer in vitro.Methods The proliferation of the ovarian carcinoma cell line HO-8910 cultured with 17-βestrogen was evaluated by the micoculture tetrazolium assay(MTT).The expression of the estrogen receptor,progesterone receptor and androgen receptor of the cell line were examined by the immunohistchemical staining.The cell apoptosis was detected by DNA agarose gel electrophoresis and deoxynucleotidyl transferase-mediated duTP nick end labeling(TUNEL).Results 48 and 72 hours later,the growth of HO-8910 cell line was inhibited by 17-βestrogen(1×10 -6～1×10 -5 mol/L).17-βestrogen in concentration of 1×10 -5 mol/L had a marked anti-proliferative effect at 21 days.There were no singnificant difference in estrogen receptor,progesterone receptor and androgen receptor of HO-8910 after treatment with 17-βestrogen.There was no evidence of apoptosis in cell line treated with 17-βestrogen.Conclusion The anti-proliferative effect of estrogen on ovarian cell is associated with the histopathological subtypes of the cancer and steroid hormone receptor.17-βestrogen can not change the expression of steroid hormone receptor and induce apoptosis in ovarian cell line.