Preparation and Identification of McAbs against Recombinant Human Cytomegavirus (rhCMV)

Aim : To obtain hybridoma cell lines secreting McAbs against HCMV. Methods: Spleen cells from Balb/c mice immunized with rhCMV gp52 protien were fused with Sp2/0 myeloma cells.The secific McAbs were screened by ELISA and identified by Western blot. Results: Four hybridoma cell lines (3E 9 , 5A 6, 4G 12 and 2H 2) secreting specific McAbs to HCMV were selected and determinded to recognize different epitops on gp52 protein. The titers of the four clones were as the following: 1∶2048,1∶1024,1∶1024,1∶512(supernant); 1×10 -7 ,1×10 -7 ,1×10 -6 ,2×10 -6 (AF).Conclusion: McAbs 2H 2 recognize one epitope on rhCMV gp52 protien , whereas 3E 9,5A 6 and 4G 12 recognize another .