Simultaneous Determination of Benzophenone-2 and Benzophenone-3 in Cosmetics by HPLC Method

Objective To establish a high performance liquid chromatography(HPLC) method for the determination of benzophenone-2 and benzophenone-3 in cosmetics.Methods The samples were treated by ultrasonic in acetonitrile-water before detected by HPLC-UV.The analytical column was Phenomenex GEMINI C18 110A(250 mm×4.6 mm,5 μm).The mobile phase was acetonitrile-water.The flow rate was 1.0 ml/min.The detection wave length was 335 nm and the column temperature was 30 ℃.Results The linear calibration curves were obtained over the range of 1-150 μg/ml for both benzophenone-2 and benzophenone-3,the correlation coefficients were more than 0.999,the detection limits were 0.15 μg/ml,the limits of quantification were 1 μg/ml.The recoveries of the two compounds were between 90% and 100%,and the relative standard deviations were less than 2.0%.Conclusion This method was simple and accurate for the simultaneous determination of the content of benzophenone-2 and benzophenone-3 in cosmetics which do not contain wax.