The Character Analysis of the Anti-oxidation and Anti-mutagenicity of the Polysaccharide from Nostoc flagelliforme

Polysaccharide of wild Nostoc flagelliforme was isolated by hot water extraction, ethanol precipitation, and proteins were removed from it by chloroform-isoamyl alcohol. One component was got through gel filtration chromatography from the purified polysaccharide. The antioxidant activity, anti-mutagenicity and the effect to the activities of antioxidant enzymes of this component were analyzed. The results showed that the polysaccharide had strong ability of scavenging·O2-, which increased with the content of polysaccharide. The scavenge rate was linearly with the conten(ty=2.991x-0.003, R2=0.998)when which was below 0.075 g/L. The polysaccharide also had obvious ability of anti-mutagenicity. It could effectively suppress the production of micronucleus, which was induced by mitomycin. The suppressing rate increased with the polysaccharide concentration and the maximum was 68.4 % in the experiment. The activities of SOD and POD were both inhibited by this polysaccharide and the inhibition was stronger to SOD.