Isolation and Preparation of Flavones from the Bran of Tartary Buckwheat Using High Speed Counter Current Chromatography

The bran of tartary buckwheat was extracted with 60% ethanol. A DA-201 macroporous resin column was applied in the purification of flavonoids from the extract. High speed counter current chromatography(HSCCC) method was used for preparative isolation and purification of rutin and quercetin from the preliminarily purified extracts. A two-phase solvent system composed of ethyl acetate, n-butyl alcohol and water(4:1:5, V/V) was used at a flow rate of 2.0 mL/min for HSCCC separ ation at a rotation speed of 850 r/min and the pooled eluates were detected at 25 4 nm. The purity of rutin and quercetin obtained was 99% and 94%, respectively, as detected by high performance liquid chromatography(HPLC). These results indicate that this method is convenient and effective and can be used to extract and purify rutin and quercetin from tartary buckwheat bran.