Study of the ratio of CD34 positive cells in bone marrow of patients with aplastic anemia

Objective:To detect the ratio of CD34 positive cells in bone marrow mononuclear cells in patients with aplastic anemia(AA),and to find the relationship between pathogenesy of AA and hematopoietic stem,progenitor cells.Methods:The ratio of CD34 positive cells in bone marrow mononuclear cells in 21 patients with AA were detected by flow cytometry(FCM),and compared with the normal control group.Results:There was a significant difference according to the ratio of CD34 positive cells in bone marrow between AA group(0.196±0.164)%and the control group(1.129±0.570)%(P0.01). Conclusion:The ratio of CD34 positive cells in bone marrow mononuclear cells is significantly reduced in aplastic anemia patients,supporting the theory of internal defection of hematopoietic stem cells.The testing can help AA diagnosis,thus worth being popularized in clinical otolaryngology.