The determination of CD_(34) positive cells in bone marrow of the patients with myelodysplastic syndrome and aplastic anemia

Objective To detect the ratio of CD_(34)~+ cells in bone marrow mononuclear cells of the pa- tients with myelodysplastic syndrome(MDS)and aplastic anemia(AA),so to explore different pathogenesis between MDS and AA.Methods To detect the ratio of CD_(34)~+ cells in bone marrow mononuclear cells of 22 MDS patients,13 AA patients and 12 normal subjects by flow cytometry(FCM).Results There was signifi- cant difference according to the ratio of CD_(34)~+ cells in mononuclear cells of bone marrow between AA group and control group.AA group and MDS-RA group,AA group and MDS-RAEB group,MDS-RA group and MDS-RAEB group(P0.05).The ratio of CD_(34)~+ cells in mononuclear cells of bone marrow was less than 0.1% in the majority of SAA patients(3/4)while it was seldom less than 0.1% in CAA patients(1/9).Conclusion The determination of CD_(34)~+ cells can be used to judge pathogenetic condition of AA patients and the prognosis of MDS patients.It also is helpful to distinguish AA and MDS.