2006•Neural Injury and Functional ReconstructionRequires access
The Expression of p15~(ink4b) on Astrocytes and Neurons of Rats after Focal Cerebral Ischemia
Huaxian Chen
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Abstract
Objective:The change of expression of p15~(ink4b) on astrocytes and neurons of rats after focal cerebral ischemia has been stuided.Methods: Ischemia was induced by temporary middle cerebral artery occlusion(MCAO).The experimental rats induced by MCAO were sacrificed on days 1,3,7 and 14 after reperfusion.The brain was taken at days 1,3,7,14 after injury,then the p15~(ink4b) of astrocytes and neurons was detected by flow cytometry.Results: Compared with that of the controls,the expression of p15~(ink4b) on astrocytes was remarkably down-regulated on the ischemic side of cerebral cortex 3,7 and 14 days after reperfusion(P 0.05).Similarly,p15~(ink4b) on neurons was significantly down-regulated on the ischemic region 14 days after cerebral ischemia(P 0.05),but lesser than that on the astrocytes(P 0.05).Conclusion: Astrocytes and neurons show a different down-regulated expression of p15~(ink4b) after MCAO in rats.The p15~(ink4b) expression on astrocytes was more remarkably down-regulated than that on neurons.