The study on the expression of Nogo-A protein in acute spinal cord injury rats treating with high dose methylprednisolone

Objective:To study the expression of Nogo-A protein in acute spinal cord injury rats treating with high dose methylprednisolone. Methods: According to Allen's model, SD rats were divided into three groups: the normal, the acute spinal cord injury (control groups) and the acute spinal cord injury supplying with methylprednisolone treatment group. The segments of T8 to T10 were injured, and then injury spinal cord was taken out at 3,7,14 days after operation, respectively. The expression of Nogo-A protein was detected by Western blot in each phase time. After staining with HE and immunohistochemistry, the morphology of injured spinal cord was observed under the microscope. Results: Nogo-A protein expressed in the three groups. After the injury, the expression of Nogo-A protein at each time phases in both control and MP groups were significantly higher than that in normal group (P0.05). During the 7th day after damage, the highest point of Nogo-A protein expression appeared in both control and MP groups, thereafter, decreased gradually. However, Nogo-A protein expression was higher than normal group at the 14th day (P0.05). There was significant difference in groups MP with high dose and control (P0.05).The expression of Nogo-A protein at each phase time in group MP was significant lower than that in control group. Conclusions: Nogo-A is a major suppressor factor of spinal cord neuron fiber regeneration after spinal cord injury, and high dose MP can inhibit the expression of Nogo-A protein obviously.