Expression of C3c and CD59 in acute spinal cord injury tissue of rats and the interference effects of methylprednisolone

Objective: To discuss the expression of C3c and CD59 in acute spinal cord injury tissue of rats and the interference effects of methylprednisolone. Methods:The acute spinal cord injury models were made in SD rats by Allen’s assay. We observed the expression sites and time points of C3c and CD59 positive reactants in methylprednisolone group and normal saline group 12 hours, 1, 3, 5, and 7 days after injury. The differences were compared between the 2 groups. Results:Positive expression of C3c and CD59 was found in the spinal cord injury tissues of the 2 groups. Positive expression of C3c in methylprednisolone group at each time point was obviously less than that in normal saline group with significant difference (P0.01). Expression of CD59 in methylprednisolone group 12 hours, 1,and 3 days after injury was less than that in normal saline group with significant difference (P0.01, P0.05, P0.05, respectively).There was no obvious difference between the 2 groups 5 and 7 days after injury. Conclusion:Positive expression of C3c and CD59 was found in the spinal cord injury tissue. Methylprednisolone can relieve secondary spinal cord injury by inhibiting the activation of the complement system.