Separation and Determination of Sucrose Esters by HPLC-ELSD

The commercial and synthesized sucrose esters were analyzed with HPLC-ELSD method.Their separation and determination were also carried out.Samples were analyzed by a hypersil C8 reversed phase column(250 mm,4.6 mm,5 μm particle size) using V(methanol)∶V(tetrahydrofuran)=90∶10 and water under gradient conditions as the mobile phase,the flow rate was 1.0 mL/min and the column temperature was set at 40 ℃.This procedure provided a complete separation and determination of sucrose,sucrose monoester,sucrose diester,fatty acid,methyl stearate and sucrose higher esters in each fraction on a single run.The described method is found to be straight forward,rapid,efficient and can be readily applied for the analysis of sucrose esters during the processes of synthesis,purification,and structure studies.