Development of a multiplex PCR assay for detection of porcine parvovirus,porcine circovirus type 2 and pseudorabies virus

A multiplex PCR assay was developed using three pairs of primers designed according to the published sequences of porcine parvovirus(PPV) ,porcine circovirus type 2(PCV2) and pseudorabies virus(PRV) ,respectively.Specific PCR products of 531bp for PPV,466bp for PCV2,and 277bp for PRV could be simultaneously amplified by the multiplex PCR.To evaluate the multiplex PCR,23 clinical samples were detected.The results showed that the multiplex PCR assay was in accordance with each single PCR.Sequence analyses and BLAST results showed that the multiplex PCR-amplified three genes sharing over 98% homology with the corresponding sequences published in GenBank.The results suggested that the established multiplex PCR assay had potential for clinic application.