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2010•Zhongguo bijiao yixue zazhiRequires access

Effect of TGF-β_1 on Airway Smooth Muscle Cells Proliferation in Different Periods of Asthmatic Rats

Peng-xiang Lin

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Abstract

Objective To investigate the effect of TGF-β1 on airway smooth muscle cells (ASMCs) proliferation in different periods of asthmatic rats. Methods The establishment of 2 week and 6 week rat model of asthma through ovalbumin (OVA) sensitization and excitation. Primary cultures of ASMCs were established for experiments. ASMCs were treated with 1 μg /L,10 μg /L and 100 μg /L TGF-β1. Proliferation of ASMCs was detected by flow cytometry and MTT colorimetric assay. Results The percentage of cells of at S phase and A value of 2 week and 6 week asthmatic ASMCs were(34. 31 ± 1. 41)% ,(35. 96 ± 3. 46)% ,(0. 546 ± 0. 005) and (0. 559 ± 0. 009) respectively,significantly increased compared with the control group〔(12. 24 ± 2. 64)% ,(0. 289 ± 0. 009)respectively,(P 0. 01)〕. The percentage of cells of at S phase and A value of 2 week and 6 week asthmatic ASMCs,which were cultured in different concentration of TGF-β1,were significantly higher than the asthmatic group(P 0. 01). The percentage of cells of at S phase and A value of 10 μg/L and 100 μg/L TGF-β1 group were significantly higher than 1 μg/L TGF-β1 group(P 0. 01); and there was no significant change in ASMCs proliferation compared 10 μg /L TGF-β1 group with 100 μg /L TGF-β1 group ( P 0. 05). There was no significant difference in the effect of TGF-β1 on ASMCs proliferation between 2 week and 6 week asthmatic model group ( P 0. 05). Conclusion Compared with normal rats,the proliferation of ASMCs in different periods of asthmatic rats were obvious and that of 6 week asthmatic model group was more obvious,the percentage of cells at S phase was increasing. And ASMCs in different periods of asthmatic rats were intervened by TGF-β1 ,the percentage of cells at S phase was increased and the proliferation of ASMCs was enhanced,TGF-β1 may contribute to the proliferation of ASMCs in the early stage of asthmatic rats,and significantly promote the proliferation of ASMCs in different periods of asthmatic rats.

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Objective To investigate the effect of TGF-β1 on airway smooth muscle cells (ASMCs) proliferation in different periods of asthmatic rats. Methods The establishment of 2 week and 6 week rat model of asthma through ovalbumin (OVA) sensitization and excitation. Primary cultures of ASMCs were established for experiments. ASMCs were treated with 1 μg /L,10 μg /L and 100 μg /L TGF-β1. Proliferation of ASMCs was detected by flow cytometry and MTT colorimetric assay. Results The percentage of cells of at S phase and A value of 2 week and 6 week asthmatic ASMCs were(34. 31 ± 1. 41)% ,(35. 96 ± 3. 46)% ,(0. 546 ± 0. 005) and (0. 559 ± 0. 009) respectively,significantly increased compared with the control group〔(12. 24 ± 2. 64)% ,(0. 289 ± 0. 009)respectively,(P 0. 01)〕. The percentage of cells of at S phase and A value of 2 week and 6 week asthmatic ASMCs,which were cultured in different concentration of TGF-β1,were significantly higher than the asthmatic group(P 0. 01). The percentage of cells of at S phase and A value of 10 μg/L and 100 μg/L TGF-β1 group were significantly higher than 1 μg/L TGF-β1 group(P 0. 01); and there was no significant change in ASMCs proliferation compared 10 μg /L TGF-β1 group with 100 μg /L TGF-β1 group ( P 0. 05). There was no significant difference in the effect of TGF-β1 on ASMCs proliferation between 2 week and 6 week asthmatic model group ( P 0. 05). Conclusion Compared with normal rats,the proliferation of ASMCs in different periods of asthmatic rats were obvious and that of 6 week asthmatic model group was more obvious,the percentage of cells at S phase was increasing. And ASMCs in different periods of asthmatic rats were intervened by TGF-β1 ,the percentage of cells at S phase was increased and the proliferation of ASMCs was enhanced,TGF-β1 may contribute to the proliferation of ASMCs in the early stage of asthmatic rats,and significantly promote the proliferation of ASMCs in different periods of asthmatic rats.

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Available abstract

Objective To investigate the effect of TGF-β1 on airway smooth muscle cells (ASMCs) proliferation in different periods of asthmatic rats. Methods The establishment of 2 week and 6 week rat model of asthma through ovalbumin (OVA) sensitization and excitation. Primary cultures of ASMCs were established for experiments. ASMCs were treated with 1 μg /L,10 μg /L and 100 μg /L TGF-β1. Proliferation of ASMCs was detected by flow cytometry and MTT colorimetric assay. Results The percentage of cells of at S phase and A value of 2 week and 6 week asthmatic ASMCs were(34. 31 ± 1. 41)% ,(35. 96 ± 3. 46)% ,(0. 546 ± 0. 005) and (0. 559 ± 0. 009) respectively,significantly increased compared with the control group〔(12. 24 ± 2. 64)% ,(0. 289 ± 0. 009)respectively,(P 0. 01)〕. The percentage of cells of at S phase and A value of 2 week and 6 week asthmatic ASMCs,which were cultured in different concentration of TGF-β1,were significantly higher than the asthmatic group(P 0. 01). The percentage of cells of at S phase and A value of 10 μg/L and 100 μg/L TGF-β1 group were significantly higher than 1 μg/L TGF-β1 group(P 0. 01); and there was no significant change in ASMCs proliferation compared 10 μg /L TGF-β1 group with 100 μg /L TGF-β1 group ( P 0. 05). There was no significant difference in the effect of TGF-β1 on ASMCs proliferation between 2 week and 6 week asthmatic model group ( P 0. 05). Conclusion Compared with normal rats,the proliferation of ASMCs in different periods of asthmatic rats were obvious and that of 6 week asthmatic model group was more obvious,the percentage of cells at S phase was increasing. And ASMCs in different periods of asthmatic rats were intervened by TGF-β1 ,the percentage of cells at S phase was increased and the proliferation of ASMCs was enhanced,TGF-β1 may contribute to the proliferation of ASMCs in the early stage of asthmatic rats,and significantly promote the proliferation of ASMCs in different periods of asthmatic rats.

Key concepts: Ovalbumin, Flow cytometry, Internal medicine, Endocrinology, Transforming growth factor, Sensitization, Medicine, Asthma

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Effect of TGF-β_1 on Airway Smooth Muscle Cells Proliferation in Different Periods of Asthmatic Rats — Research Paper | ScholarLens