Effects of Rho-kinase inhibitor Y-27632 on the proliferation and cell cycle of human airway smooth muscle cells

Objective To investigate the effects of Rho-kinase inhibitor Y-27632 on the proliferation of human airway smooth muscle cells(ASMCs),so as to provide new ideas for the treatment of asthma.Methods The ASMCs were primarily cultured to log phase by explant method and then divided into four groups,group A were treated with 10%FBS+DMEM,group B,C,D were treated with Y-27632 at the concentration of 0.4,2.0,10.0 μmol/L respectively +10%FBS+DMEM.MTS/PMS assay was used to investigate the proliferation of ASMCs(A Value at 490 nm).Flow cytometry was used to analyze the cell cycle.Results The A Value of group C and D were significantly lower than that of group A(P0.05,0.01).The cell percentages of G0/G1 phase of group C and D were much higher than that of group A,while cell ratios in S and G2/M were lower than that of group A(P0.05,0.01).Conclusions Rho kinase inhibitor Y-27632 can inhibit the proliferation of ASMCs.This study provides new options for the treatment of asthma.