HSP47 is involved in TGF-β1-induced collagen expression in human embryonic lung fibroblast

Objective By investigating effect of the dynamic expression of HSP47 in human embryonic lung fibroblast(HELF) on pulmonary fibrosis to explore the relation between HSP47 and pulmonary fibrosis.Methods Dynamic expression of HSP47,CollageⅠ,α-SMA and Vimentin were observed after HSP47 was induced to express in HELF with incubation of TGF-β1.HELF was stimulated by TGF-β1(5 ng/mL) at different time points and different dosages for 48 h respectively.The proliferative rate of HELF was tested by WST.Expression of HSP47 was determined by immunofluorescence.Expressions of HSP47,CollageⅠ,α-SMA and Vimentin were detected by Western blot.ResultsHSP47 showed slight expression in normal HELF.Expressions of HSP47(5 ng/mL TGF-β1 48 h,7.081±0.265),CollageⅠ(5 ng/mL TGF-β1 48 h,2.494±0.139),α-SMA and Vimentin increased synchronously by means of time-independence and dosage-independence after HELF being treated by TGF-β1(P0.05).ConclusionHSP47 participated in the expression of collagen in human embryonic lung fibroblast induced by TGF-β1,suggested that HSP47 might play an important role in pulmonary fibrosis as collagen-specific molecular chaperone.