Effect of TGF-β_1 on heat shock protein 47 expression of human renal fibroblasts cultured in vitro

Objective To investigate the mechanism of TGF-β1 action in chronic renal fibrosis.Methods Human renal fibroblasts primarily cultured in vitro were divided into 5 groups:(1) the control group with 0ng/ml TGF-β1;(2) 0.1gng/ml TGF-β1 trial group;(3)1ng/ml TGF-β1 trial group;(4)5ng/ml TGF-β1 trial group;(5)10ng/ml TGF-β1 trial group.Expression of heat shock protein 47(HSP47),which in human renal fibroblasts in vitro under the effect of TGF-β1,was determined by immunocytochemistry.And expressions of HSP47 mRNAs and I collagen mRNAs were examined by semi-quantitive reverse transcriptase polymerase chain reaction(RT-PCR).Results Compared with the control group,expression of HSP47 and collagen I in cultured human renal fibroblasts significantly increased in the trial groups.TGFβ1 promoted expression of collagen I and HSP47 of renal fibroblast in dose-dependent manner range from 0.1ng/ml to 5ng/ml.Conclusion TGF-β1 is involved in occurrence and development of renal fibrosis partly by enhancing expression of HSP47 in renal fibroblasts and further increasing collagen synthesis.