Effect of Fluoride on the changes of ultrastructural and cell cycle in fibroblast

Objective To study the effects of fluoride on the changes of ultrastructure and cell cycle in fibroblast.Methods Fibroblasts were exposed to different concentrations of fluoride(0,0.000 1,0.001,0.01,0.1,1,10 and 20 mg/L F~-) for 48 h.The ultrastructure was observed by electron microscope.DNA synthesis and cell apoptosis were studied by using flow cytometry.Results Some findings including lots of enlarged rough endoplasmic reticula,Golgi complexes,fat drops and glycogen were observed in the fluoride-treated groups.No obvious cytotoxicity was found.The percentage of cell in G0-G1 phase was 54.0% in the group of 0 mg F~-/L and 48.8% in the group of 0.1mg F~-/L;the percentage of S phase was 31.7% in the group of 0mg F~-/L and 32.4%,46.1%,32.4%,35.1%,and 33.7% in the groups of 0.000 1,0.001,0.1,1,10 and 20mg F~-/L respectively.The percentage of apoptotic cell was 3.1% in the group of 0 mg F~-/L and 2.6%,2.5%,1.3%,(0.8%,) 0.5%,1.9% in the groups of 0.000 1,0.001,0.1,1,10 and 20 mg F~-/L respectively.Conclusions The results suggest that fluoride can stimulate the functions of fibroblasts in cell proliferation,differentiation,synthesis,secretion and inhibit the cell apoptosis significantly.