SYNERGISTIC ANTI-PROLIFERATIVE EFFECT OF TRICHOSTATIN A AND 5-FLUOROURACIL ON HUMAN HEPATOMA CELL LINE HEPG2

[Objective]To investigate the growth-inhibitory effect of trichostatin A(TSA)or(and)5-fluorouracil(5-FU)against the proliferation of human hepatoma cell line HepG2 and the expression of NF-κB p65 and AKT.[Methods]MTT assay was used to observe the effects on growth inhibition induced by TSA and 5-FU in HepG2 cells.Apoptosis was observed by Hoechst 33258 staining and the levels of NF-κB p65 protein and AKT protein were detected by Western blot.[Results]Compared with control group,the proliferation of HepG2 cell was inhibited in a dose-dependent manner after the treatment of TSA or 5-FU.Treated with TSA and 5-FU in combination,the inhibition and apoptosis can significantly increase,while the expression of NF-κB p65 protein and AKT protein reduced(P﹤0.01).[Conclusion]5-FU combined with TSA can inhibit the proliferation of hepatoma cell HepG2,which maybe related to the downregulation of NF-κB p65 protein and AKT protein.