Study of effect of TSA on human hepatocellular carcinoma cells

Objective:To investigate the mechanism of trichostatin A (TSA) by comparing the effects of TSA on the proliferation and apotosis of human hepatocellular carcinoma HepG2 cells and normal hepatocellular LO2 cells. Methods:MTT,phase -contrast -microscope,electron -microscopy, immunocytochemistry were conducted to observe the alteration of cell proliferation, apotosis and apoptosis-related protein of HepG2 and LO2 after treated with TSA.Results:The HepG2 cells altered in their cells forms and the early apoptosis was observed under electron microscope. Low dosage TSA had a very strong inhibition to HepG2 cells, nearly no efficacy on LO2 cells. When the TSA concentration of 1000nmol/L or above,the apparent cytotoxicity appeared to LO2 cells. TSA could induce the apoptosis of HepG2 cells and increase the expressions of bax. Conclusion:TSA can inhibit the proliferation of HepG2 cells. This maybe related to cell apoptosis by upmodulating the expression of Bax.