Pharmacokinetics of Xiangbei Injection in Hybrid Dogs by HPLC-MS Assay

Objective To study the pharmacokinetics of Xiangbei Injection after intravenous administration in hybrid dogs.Methods The plasma concentrations of 4,5-dimethoxy-canthinone were determined by HPLC-MS assay in two groups of hybrid dogs with intravenous injection of Xiangbei Injection through the femoral vein at the doses of 0.8,1.6 mL·kg-1,respectively.The practical pharmacokinetics software 3p97 was used for the calculation of pharmacokinetic parameters.Results The kinetic process of Xiangbei Injection in hybrid dogs was fitted to the two-compartment model.The pharmacokinetic parameters for dogs given the doses of 0.8 mL·kg-1 and 1.6 mL·kg-1 were as follows:the half life of distribution(t1/2α)were 1.15 min and 1.24 min,half life of elimination(t1/2β)were 8.69 min and 9.85 min,area under curve(AUC)were 124.27 ng·mL-1·min-1 and 234.78 ng·mL-1·min-1,and plasma clearance rate(CL)were 450.64 mL·min-1 and 477.04 mL·min-1,respectively.Conclusion Xiangbei Injection is quickly distributed in hybrid dogs after intravenous administration,and the concentration decreases rapidly.