EFFECTS OF YISHEN DECOCTION AND WESTERN MEDCINE COMBINED APPLICATION ON RENAL TISSUE TGF-β_1 EXPRESSION IN MICE WITH IgA NEPHROPATHY

Objective:To discuss the mechanism of yishen decoction on IgA nephropathy.Methods:IgA nephropathy models were built by intaking bovine serum albumin(BSA) with injecting staphylococcus enterotoxinB(SEB) through caudal vein.The TGF-β1mRNA expression was determined by fluorescent quantitative polymerase chain reaction(FQ-PCR) and the content of TGF-β1 protein was tested by immunohistochemistry.Results:No significant difference in mRNA and protein expression of TGF-β1 was found between the low concentration and high concentration yishen decoction groups,but the TGF-β1mRNA expression and its protein secretion in the two concentration yishen decoction were lower than those in the IgA nephropathy model group,which were the lowest in the western medicine combined yishen decoction group among the five treatment groups.Conclusion:The therapeutic effects of yishen decoction on IgA nephropathy may be associated with the inhibition of TGF-β1mRNA expression and its protein excretion.