Determinnation of Antioxidant Capacity of Vatica mangachapoi accelerated solvent extraction(ASE) with DPPH Assay

Objective:To investigate the antioxidant effect of medicinal plants Vatica mangachapoi in Hainan province.Method: The sample extractions were prepared by accelerated solvent extraction(ASE) using solvent petroleum ether(MSO),ethyl acetate(EtOAc),ethanol(EtOH),methanol(MeOH);Combining with microplate assay model,the ASEs that had high antioxidant activity were screened quickly.Result: The antioxidant activity of different solvent ASEs from Vatica mangachapoi was evaluated by DPPH assay.The antioxidant activity was listed as follows: ethyl acetateethanolpetroleum ethermethanol;its IC50 values were 0.143 4,0.501 8,0.567 7,0.693 7 g·L-1 respectively.The antioxidant of ethyl acetate ASE was better than ascorbic acid(IC50=0.203 4 g·L-1).Conclusion: The ethyl acetate ASE showed great DPPH scavenging activity,it's hopeful to develop a new natural antioxidant.