Studies on Degranuation of Rat Peritoneal Mast Cells and RBL-2H3 Cells Caused by Traditional Chinese Medicine Injections

Objective: Evaluating the feasibility of the degranulation methods in rats mast cells in vitro and in RBL-2H3 cells in vitro caused by traditional Chinese medicine injections(TCMI).Method: Mast cells were gathered from rats peritoneal,the experimental conditions were optimized,the positive drug C48/80 and various TCMI were incubated to observe rat peritoneal mast cells degranulation caused by TCMI,through counting the degranulation rate of rat peritoneal mast cells whitch staining by toluidine blue.The positive drug C48/80 and various TCMI with RBL-2H3 cells were incubated to,detect the release rate of β-hexosaminidase by the method of substrate's colouration.Result: It showed certain concentration-response relations between the concentration of positive drug C48/80 and the degranulation rate of rat peritoneal mast cells;Shenmai,Guanxinning,Tanreqing,Qingkailing and Shengmai,total significantly caused mast cell degranulation,while the degranulation of mast cell and the concentration of drug had a certain positive correlation;and Shenmai,Huangqi,Yinxing,Tanreqing,Danxiang,Guanxin,Qingkailing,Shengmai which the release rate of β-hexosaminidase highen by RBL-2H3 cells.Conclusion: Some TCMI can cause rat peritoneal mast cells and RBL-2H3 cells degranulation in vitro,so they have certain potential application values and complementarity with the two methods evaluting anaphylactoid reaction caused by traditional Chinese medicine injection.