ScholarLens Back to search
2003•Tianjin Yike Daxue xuebaoRequires access

Cloning of IGF1 gene containing signal peptide coded sequence

Jingyu Zhang

Open publisher page 0 citations

Abstract

Objective:To Cloning IGF-1gene which contained not only mature but also signal peptide coded sequence as fundament for study the trans IGF-1gene.Methods:Amˉplified mature and signal peptide coded sequence of IGF-1gene respectively.Connected these two DNA fragments together by PCR and inserted the newly formed360bp DNA fragment into plasmid pGEM-Teasy.Results:The recombinant plasmid contained an exogenous DNA fragˉment of360bp encoding IGF-1signal and mature peptide.Conclusion:The expression of IGF-1gene and a more simple and economic competitive PCR assay for already cloned gene is put forward.

About this research paper

What this paper is about

Objective:To Cloning IGF-1gene which contained not only mature but also signal peptide coded sequence as fundament for study the trans IGF-1gene.Methods:Amˉplified mature and signal peptide coded sequence of IGF-1gene respectively.Connected these two DNA fragments together by PCR and inserted the newly formed360bp DNA fragment into plasmid pGEM-Teasy.Results:The recombinant plasmid contained an exogenous DNA fragˉment of360bp encoding IGF-1signal and mature peptide.Conclusion:The expression of IGF-1gene and a more simple and economic competitive PCR assay for already cloned gene is put forward.

Why it matters

A significance statement is not available in the OpenAlex record.

Key contribution

A contribution statement is not available in the OpenAlex record.

Method / approach

Method details are not available in the OpenAlex metadata.

Main findings

Findings are not separately available in the OpenAlex metadata.

Limitations

Limitations are not available in the OpenAlex metadata.

Applications

Application details are not available in the OpenAlex metadata.

Available abstract

Objective:To Cloning IGF-1gene which contained not only mature but also signal peptide coded sequence as fundament for study the trans IGF-1gene.Methods:Amˉplified mature and signal peptide coded sequence of IGF-1gene respectively.Connected these two DNA fragments together by PCR and inserted the newly formed360bp DNA fragment into plasmid pGEM-Teasy.Results:The recombinant plasmid contained an exogenous DNA fragˉment of360bp encoding IGF-1signal and mature peptide.Conclusion:The expression of IGF-1gene and a more simple and economic competitive PCR assay for already cloned gene is put forward.

Key concepts: Signal peptide, Cloning (programming), Recombinant DNA, Plasmid, Gene, DNA, Molecular cloning, Molecular biology

Related papers

Related paper 1Related paper 2Related paper 3
Back to paper searchBrowse research topicsOriginal source
Cloning of IGF1 gene containing signal peptide coded sequence — Research Paper | ScholarLens