Gene cloning and construction of prokaryotic expression vector of Human β2-microglobulin chain mature peptide gene

Two pairs of primers were designed according to the reference Human β2m mature peptide genes from GenBank.The Human β2m mature peptide gene was amplified from the blood of healthy people by using RT-PCR.PCR product was cloned into the T easy vector and sequenced.The sequencing result showed that the target gene was 297 bp and the homology between the Human β2m mature peptide gene and the template is 100%.The Human β2m mature peptide gene was subcloned into pET-28a(+) vector.The recombinant plasmid pET-28/ Hum β2m was identified by PCR,DNA restriction and sequencing.The result showed that the recombinant plasmid pET-28/ Hum β2m was constructed correctly and successfully.