Screening and Study on Fermentation Conditions of Endophytic Antifungal Bacteria Isolated from Pear Plant

With Alternaria alternata as indicator,the strain LP5 was screened from eight strains of endophytic Bacillus sp.,which were isolated from xylem of pear plant.To confirm the optimal components and cultural conditions of strain LP5,the antifungal activity and OD600 of the strain LP5 were studied by the method of single factor and orthogonal design.Results showed that the optimal cultural liquid medium was composed of 1.5% sucrose,1.0% protein peptone,0.5% gyeast extract,0.5% NaCl,and the optimal fermentation conditions were 30 ℃,pH 7.0 and culturing for 48~72 h.