Inhibitory effect of novel polyoxometalate on hepatitis B virus replication in vitro

Objective To evaluate the activity of novel polyoxometalate against hepatitis B virus (HBV) in vitro.Methods MTT assay was used to evaluate the growth inhibitory effects of polyoxometalate on 2.2.15 cell lines,Hepatitis B e(s) antigen(HBeAg) and Hepatitis B surface antigen in the culture medium were determined by ELISA.Southern blotting was performed to detect HBV DNA in Hep G2 2.2.15 cells.Real-time quantity PCR was applied to detect the contents of introcellular HBV mRNA and extracellular HBV DNA.Results The median toxicity concentration(TC50) was 1590.46 mg·L-1 on 2.2.15 cells.The inhibitory rats of PTW-6 on HBeAg and HBsAg were markedly higher compared with control group(P0.05).The median inhibitory concentration(IC50) of PTE-6 in extracellular HBV DNA and introcellular HBV mRNA were 51.1 and 63.6 mg·L-1,respectively.Conclusion Novel polyoxometalate is a potential compound for treatment chronic HBV infection.