Construction of complete S100A2 eukaryotic expression vector and its expression in BGC-823 cell line

Objective To construct an eukaryotic expression vector of human S100A2 full length gene named pEGFP-N2-S100A2 and transfect it into gastric cancer cell line BGC-823.Methods The complete gene of S100A2(294 bp) was obtained by RT-PCR using human gastric epithelial tissue mRNA as template.The PCR product was connected into pEGFP-N2 vector by gene recombinant technique.The plasmid was detected by restrictive enzyme digestion and PCR,and sequenced right plasmid was transfected into BGC-823 cells using lipid reagent.Results The cloning of the complete S100A2 gene and the construction of expression vector were accomplished by gene recombinant technique.The sequence analysis suggested that the S100A2 sequence was identical to that published in GenBank(NM-005978).The expression of green fluorescence protein(GFP) was observed under the fluorescence microscope.Conclusions The eukaryotic expression vector pEGFP-N2-S100A2 is constructed and expressed successfully in BGC-823 cells.