Construction of the eukaryotic expression vector of human SET gene and its expression in L-02 liver cells

Objective To construct a recombinant plasmid carrying enhanced green fluorescent protein and the inhibitor of protein phosphatase 2A (SET) and detect its expression in L-02 liver cells. Methods The SET cDNA of human L-02 liver cells was cloned by RT-PCR and the eukaryotic expression vector of SET gene was constructed by gene recombination technique. The recombinant plasmid pEGFP-N2-SET was verified by restriction enzyme digestion analysis and sequenceing, then transfected into human L-02 liver cells using LipofectamineTM 2000. Then the expression level of the fusion protein was detected by fluorescence microscope. Results The eukaryotic expression plasmid pEGFP-N2-SET was successfully constructed. The SET gene was expressed successfully in transfected L-02 liver cells. Conclusion The successful construction of and expression of SET recombinant palsmid has set foundation for further investiagtion of its structure and function.