Extraction Optimization of Total Flavonoids from the Flowers of Dendranthema morifolium (Ramat) Tzvel.cv.Chuju

Pure water and aqueous solutions of ethanol at different concentrations were used to extract total flavonoids from the flowers of Dendranthema morifolium(Ramat) Tzvel.cv.Chuju at 60 ℃,respectively,and ethanol aqueous solution was the selected solvent for total flavonoids extraction based on experimental results.This was followed by the optimization of the process for total flavonoids extraction using orthogonal array design.The results indicated that the optimum extraction conditions were determined as follows:ethanol concentration 80%,extraction temperature 70 ℃ and solid-to-liquid ratio 1:100.Among these conditions,solid-to-liquid ratio exhibited the most significant effect on total flavonoids extraction.