2003•Unpublished venueRequires access
The difference in acute lung injury caused by intratracheal instillation or intraperitoneal injection of LPS
Hong Li
Open publisher page 1 citations
Abstract
Objective:To investigate the difference in acute lung injury caused by pulmonary and extrapulmonary factor Methods: A rat model of ALI was induced by intratracheal(IT) instillation or intraperitoneal(IP) injection of LPS with a dose of 10mg/kg 54 male SD rats were randomly divided into control group?IT group and IP group,every group included 18 rats(6 rats at 2h?6h and 24h respectively) the arterial gas analysis?the total protein concentration?the total cell counts and classification?the level of TNF α in BALF?lung pathology of rats were examined Results: Comparision with IP group,in ITgroup the PaO 2 was decreased sharply( P 0 001), the total protein concentration?the total PMN in BALF?the level of TNF αin BALF were greatly increased( P 0 001or P 0 05), lung pathology showed severe polymorphonuclears infiltration with bleeding and hyaline membranes Conclusion: ①PaO 2 of rats in IT group decreased progressively,intraperitoneal injection of LPS has no effcct on PaO 2 of rats ②Overwhelming PMN infiltration was found in rats of IT group and was more severe than IP group