Study about heparin reduces acute lung injury of rats induced by endotoxin

Objective To investigate the effect of different dose of heparin reduces acute lung injury in rats induced by Lipopolysaccharide (LPS).Methods LPS (5mg·kg-1) was infused by femoral vein to reproduce ALI model in rats.Fifty male SD rats were randomly divided into five groups with 10 animals in each group:in group C1-C3 LPS 5mg·kg-1 and heparin 250u·kg-1,500u·kg-1 and 1000u·kg-1 was infused by femoral vein simultaneously;group B received LPS 5 mg/kg;group C served as control one by giving the same volume of saline.At 4 h after LPS was given,and then the animals were killed.The pathological manifestation of lungs were observed,and the lung W/D ratio was calculated,and the concentration of malondialdehyde (MDA) in lung was detected,the concentration of TNF-a and protein in BALF were detected.Results Obvious ALI pathological manifestation in the lungs of B group rats were observed.Comparing with A group,the lung W/D ratio and concentration of TNF-a and protein in BALF significantly increased in B group (P0.01) as well as the concentration of MDA activity in lung (P0.01).Comparing with LPS group,the pathological manifestation in the lung and the lung W/D ratio and concentration of TNF-a and protein in BALF and the concentration MDA decreased in C1-C3 group (P0.05).However,bleeding complications increased with the dose of heparin increased.Conclution Early treatment of heparin can provide evident effects on ALI in rats,250u·kg-1 was the best treatment dose.