Expression and significance of the Bim-1 gene in gastric carcinoma

Objective:To detect the expression of Bim-1 gene in gastric carcinoma,adjacent non-cancerous tissues and the peripheral blood of the corresponding gastric carcinoma patient,to explore whether its expression is correlated with the clinical pathology of the gastric carcinoma.Methods:The expression of Bim-1 gene was detected by reverse transcriptase polymerase chain reaction(RT-PCR) and real-time PCR in gastric carcinoma,and adjacent non-cancerous tissues from 80 patients and the gastric carcinoma cell strain(SGC-7901,BGC-823,AGS).Furthermore,we detected at the same time the expression of Bim-1 gene in the peripheral blood of both 20 corresponding gastric carcinoma patients and health people.Using real-time PCR and nested RT-PCR detected the expression of Bim-1 gene in the peripheral blood.Western blot were employed to detect Bim-1 protein level.Results:The positive rates of Bim-1 expression was found in gastric tumor tissues was 85% (68/80),which was significantly higher than those was found in the matching,adjacent non-cancerous tissues 30% (24/80) (P0.05),A high level of Bim-1 expression was found in the gastric carcinoma cell strain(SGC-7901,BGC-823,AGS).In addition,The positive rates of Bim-1 expression in gastric carcinoma patient was higher than the healthy people.Bim-1 expression at the transitional levels were markedly different between gastric carcinoma and adjacent non-cancerous tissues by Western blot analysis.Conclusion:Bim-1 expression may play an important role in the development of the gastric cancer.It may be a novel diagnostic marker of human gastric cance.