Effect of everolimus on the neointimal hyperplasia of carotid artery in rats injured by balloon

Objective:To study the effect and analyse the possible mechanism of oral everolimus on the neointimal hyperplasia of carotid artery in rat models injured by balloon. Method:Thirty-six healthy male SD rats were randomly divided into the sham-operated group(n=12),the injury group(injured carotid artery by balloon,n=12) and the everolimus group(injured carotid artery by balloon+oral everolimus,n=12).1.5 mg/kg Everolimus was orally administrated 1 day before injuring carotid artery by balloon followed by 0.75 mg/kg per day for 28 days.The control groups were given with matching equivalent normal saline.Drugs were administered by oral gavage.Carotid tissues of three groups were harvested 28 d after carotid artery injury.The target vascular sections were stained with HE to observe the morphology changes.The expression of eukaryotic translation initiation factor 4E(eIF-4E) and proliferating cell nuclear antigen(PCNA) in arterial wall was detected by immunohistochemistry. Result:No neointima hyperplasia was found in the sham-operated group.The expression of eIF,-4E and PCNA in positive cells were scarce.Compared with the sham-operated group,the neointima was formed and thickened distinctly,the expression of eIF-4E and PCNA positive cells were significantly increased in the injury group.Compared with the injury group,the neointimal area decreased,the expression of eIF4E and PCNA significantly decreased in the everolimus group(P0.05). Conclusion:Everrolimus can inhibit neointimal growth in rat models with injured carotid artery by balloon.The possible mechanism is connected with inhibition the expression of eIF-4E and PCNA.