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2004•Journal of Shandong UnivenityRequires access

Early activation and activated potential of T lymphocytesin patients with aplastic anemia

Li-Zhen Li

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Abstract

Objective: To investigate the expression of T cell early activation marker(CD69) on CD4+ and CD8+ lymphocytes in peripheral blood of patients with aplastic anemia (AA) and the pathophysiological significance of the changes in CD69 expression in different T cell populations of both pre-and post-culture in vitro. Methods: The whole blood cell culture procedure was applied to activate T lymphocytes by PHA (20 μg/ml) in vitro. The expression rates of CD69 of CD4+ and CD8+ lymphocytes 0 h and 4 h after culture were analyzed by two-color flow cytometry. Results: The expression rates of CD69 of CD4+ and CD8+ cells in patients with SAA were(8.968)% respectively, and the expression rate of CD69 of CD8+ cells in patients with CAA was(7.3648.78or role in the pathogenesis of AA.

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Objective: To investigate the expression of T cell early activation marker(CD69) on CD4+ and CD8+ lymphocytes in peripheral blood of patients with aplastic anemia (AA) and the pathophysiological significance of the changes in CD69 expression in different T cell populations of both pre-and post-culture in vitro. Methods: The whole blood cell culture procedure was applied to activate T lymphocytes by PHA (20 μg/ml) in vitro. The expression rates of CD69 of CD4+ and CD8+ lymphocytes 0 h and 4 h after culture were analyzed by two-color flow cytometry. Results: The expression rates of CD69 of CD4+ and CD8+ cells in patients with SAA were(8.968)% respectively, and the expression rate of CD69 of CD8+ cells in patients with CAA was(7.3648.78or role in the pathogenesis of AA.

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Available abstract

Objective: To investigate the expression of T cell early activation marker(CD69) on CD4+ and CD8+ lymphocytes in peripheral blood of patients with aplastic anemia (AA) and the pathophysiological significance of the changes in CD69 expression in different T cell populations of both pre-and post-culture in vitro. Methods: The whole blood cell culture procedure was applied to activate T lymphocytes by PHA (20 μg/ml) in vitro. The expression rates of CD69 of CD4+ and CD8+ lymphocytes 0 h and 4 h after culture were analyzed by two-color flow cytometry. Results: The expression rates of CD69 of CD4+ and CD8+ cells in patients with SAA were(8.968)% respectively, and the expression rate of CD69 of CD8+ cells in patients with CAA was(7.3648.78or role in the pathogenesis of AA.

Key concepts: CD69, Aplastic anemia, Flow cytometry, CD8, Pathogenesis, Immunology, In vitro, T cell

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