Cytotoxic Activities to Tumour Cells of CIK Cells from Patients with Different Course of CIK Treatment

Objective To investigate the differences in anti-tumor effect of CIK cells in vitro among cancer patients with different course of CIK treatments.Methods Peripheral blood mononuclear cells(PBMC)were separated from cancer patients with multi-course and single-course CIK treatments,and then were induced by cytokines.Tumor cell killing activity was detected by MTT assay.Surface antigens expression was detected by flow cytometry.Results CIK in patients with different course of CIK treatment had cytotoxic activity on tumor cells to a certain extent.When effector target ratio was 4:1,8:1 and 16:1,the cytotoxicity to BGC-823 and SPC-A-1 of CIK cells from patients with multiple courses treatment was stronger than single course treatment.When effector target ratio was 4:1,the difference in the cytotoxicity to SPC-A-1 of CIK cells was more obvious.In addition,CD3+CD56+ cells of patients with multiple treatment group were significantly higher than patients with the single treatment(P0.05).Conclusion The cytotoxic activities to tumor cells of CIK cells from multiple treatment patients were higher than those of single treatment patients.This indicats that repeated treatment can prolong survival of tumor patients,which can improve the quality of life and has a good effect in prevention of tumor recurrence.