Influence of Huanglian Jiedu Decoction on NF-κB and TNF-α of Liver in Diabetic Rats

Objective:To study the influence of Huanglian Jiedu Decoction on NF-κB,TNF-α of liver in diabetic rats,explore therapeutic mechanism of Huanglian Jiedu Decoction on diabetes.Method: The rat diabetic model was made by high fat diet and small doze of streptozotocin(STZ).After giving Huanglian Jiedu Decoction(11.5,5.7,2.85 g.kg-1)for 8 weeks,FGB,CHO,TG,LDLC and HDLC were measure,the liver and pancreas mass indexes were calulated.Using the immunohistochemical methods the contents of NF-κB and TNF-α in the liver of rats were determined.Result: High-dosage group and middle-dosage group of Huanglian Jiedu Decoction could significantly decrease the contents of CHO,TG,LDLC in rat serum,increase the contents of HDLC,lower the liver and pancreas mass indexes obviously.The expression of NF-κB,TNF-α in the liver was lower in the treatment groups compared with that in the model group.Conclusion: Huanglian Jiedu Decoction has therapeutic effect on diabetic rats induced by high fat diet and small doze of streptozotocin.The mechanism might be related to regulating the expression of NF-κB,TNF-α,inhibiting inflammatory signal pathway.