The detection of gene polymorphisms of MTHFR C677T and A1298C in severe depression patients

Objective Investigating the relationships of polymorphisms of 5,10-MTHFR gene and severe depression in northern Chinese Han population to provide evidences for the prevention and cure of diseases.Methods Using case-control study,we detected the gene polymorphisms of MTHFR C677T and A1298C by polymerase chain reaction-restriction fragment length polymorphism(PCR-RFLP).Results(1)The frequency of 677TT in control groups and that of T alleles were 13.16% and 39.80%;the frequencies of genotypes of 1298CC and that of C alleles were 1.32% and 12.83%;(2)The frequency of MTHFR 677T/T genotype(35.53%) and 677T allele(57.24%) in depression was respectively higher than that in control group(13.16%)(P0.001);the differences of the frequencies of MTHFR A1298C genotype and C allele between control group were respectively no statistically significant(P0.05);(3)The Logistic regression analysis showed that the genotype of C677T was relative to the occurrence of disease,and the genotype of A1298C was not relative to the occurrence of disease.Conclusion Themutation of MTHFR C677T in severe depression patients was relative to the occurrence of severe depression disease,and it is the risk factors of disease;the mutation of MTHFR A1298C was not relative to the occurrence of the severe depression.