2004•Zhonghua putong waike zazhiRequires access
Expression of macrophage inflammatory protein-2 in liver injury induced by ischemia and reperfusion in rat
Wen Zhang
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Abstract
Objective To study the expression and effect of macrophage inflammatory protein-2(MIP-2) in liver injury induced by ischemia/reperfusion(I/R). Methods Thirty-two rats were randomly divided into 4 groups(8 rats in each group):false operation (control) group and 3, 9, 24 hours reperfusion group.The expression of MIP-2 mRNA in hepatic tissue, MIP-2 protein in plasma, the neutrophil infiltration in liver tissue and serum ALT were measured. Results The expression of MIP-2 mRNA in the ischemic tissue was significantly higher than that in nonischemic tissue (P0.01), and was significantly higher in 9 hours reperfusion group than that in 3 hours reperfusion group (P0.01); significantly higher in 24 hours reperfusion group than that in 9 hours reperfusion group (P0.01). The level of plasma MIP-2 protein, the neutrophil infiltration in liver, the degree of liver injury had the similar changs of MIP-2 mRNA.The expression of MIP-2 mRNA in liver ischemia tissue and the level of plasma MIP-2 protein had positive correlation with neutrophil infiltration in liver tissue(P0.01) . Conclusions The results suggeste that MIP-2, one of the neutrophil chemotactic factors, plays an important role in the liver injury induced by I/R in rats.