The immunophenotype analysis of myelodysplastic syndrome

Objective:To explore the immunophenotype characteristics of myelodysplastic syndrome,and evaluate the value of flow cytometry in the diagnosis of MDS.Method:To detect the immunophenotype of the mononuclear cells of the bone marrow in 20 MDS patients and 10 controls by flow cytometry.Result:①The proportion of CD34+ cells in MDS was increased,and the proportion in hi-grade MDS was higher than that in low-grade MDS,while there was no significant difference between low-grade MDS and control.②The mean fluorescence intensity of side scatter in myeloid cells was lower than that in the control.③The MDS patients showed significantly abnormal distribution pattern of CD13/CD16 compared to the control,which was independent to the morphology of myeloid dysplasia.④The frequency of CD56 was expressed higher in MDS than that in control.⑤The means fluorescence intensity of CD71 in the erythroid cells was lower than that in control.⑥The MDS show a significant abnormal distribution pattern of CD71/GlyA compared to the control,which is independent to the morphology of erythroid dysplasia.Conclusion:FCM is significant in the diagnosis of MDS.