Immunophenotype features of CD34 positive cells in patients with myelodysplastic syndrome

Objective To explore the immunophenotype features of CD34 positive cells in patients with myelodysplastic syndrome(MDS).Methods The immunophenotypes of the CD34 positive cells in bone marrow nuclear cells of 20 patients with MDS were performed with a panel of monoclonal antibodies and four-color flow cytometry by secondary gating strategy.Results With the progression of MDS from RA/RAS,RAEB to RAEBT,the proportion of CD34~+ cells was gradually increased from 7.6%,40.1% to 71.3%(P0.05).Using CD45 vs CD34 gating strategy,the expressions of HLA-DR,CD13,CD33,CD117 on blasts were higher by secondary gating method.However,there were no differences in the expression of above-mentioned antigens on CD34~+ cells in different MDS subtypes(P0.05).Conclusion Secondary gating strategy could accurately reflect the immunophenotype features of blasts in MDS.