Detection of serum survivin in gastric carcinoma patients and its clinical significance

Objective To analyze feasibility of amplifying survivin gene in diagnosis and metastasis detection of gastric cancer.Methods Real-time quantitative reverse transcriptase polymerase chain reaction(RT-PCR) was used respectively to detect the peripheral blood carcinoma gene in normal adults,patients with gastric precancerous lesions and with gastric cancer(metastased group and non-metastased group).To evaluate the correlation between gene expression and metastasis in gastric cancer.Results In the specimens of normal adults,patients with gastric precancerous lesions and with gastric cancer,the expression rates of surviving mRNA were 0,20% and 88.46%.The expression of survivin was statistically associated with lymph node involvement and histological grades.Five-year survival rate was lower in patients with higher expression rates.Cox stepwise proportional hazards analysis showed that only and surviving status retained significant independently in prospecting prognosis.Conclusions Survivin gene was overexpressed in gastric cancer.It may be also an important marker of metastasis.RT-PCR can be used to detect metastases of gastric cancer for early diagnosis.