Effect of AduoLa Fuzhenglin on expression of caspase-3 induced by microwave exposure in rat myocardial cells

Objective To investigate the effect of an antiradiation herb compound AduoLa Fuzhenglin(ADL) on the caspase-3 expression induced by microwave exposure in myocardial cells of rats.Methods One hundred and forty male Wistar rats were divided randomly into five groups: normal control,microwave radiation control,0.75,1.5 and 3 g/(kg·d) ADL groups.Rats in three ADL groups were administrated with ADL per day for 2 weeks before microwave exposure.After administration,rats were exposed to 30 mW/cm2 microwave for 15min.Immunohistochemistry,Western blot and image analysis were used to detect the caspase-3 in rat myocardial cells 7 d and 14 d after drug withdrawal by light microscope and electron microscope.Results At 7 d and 14 d after exposure,the caspase-3 was situated in the endochylema of rat myocardial cells,and the protein expression increased significantly(P0.01) in radiation control group.The caspase-3 expression of rat myocardial cells in 0.75 g/(kg·d) ADL group was similar to that of radiation control group.The protein expression of the caspase-3 in 1.5 and 3 g/(kg·d) ADL groups decreased strikingly compared with radiation control group(P0.01).Conclusion Microwave radiation 30 mW/cm2 can cause the high expression of caspase-3;ADL in the 1.5 and 3 g/(kg·d) groups can reduce the rise of expression of caspase-3 induced by microwave.ADL can relieve rats′ heart injury by down-regulating caspase-3.