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Molecular biology identification of Bacillus sp. B_(15) and breeding of protease high-yielding strain

Li Jinhua

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Abstract

Bacillus sp.B15which produces protease,amylase and antagonistic substance was identified by 16S rDNA sequence analysis.The homology of 16S rDNA sequence of the strain B15was 99% compared with Bacillus amyloliquefaciens subsp.plantarum AS43.3,and the strain B15was identified as Bacillus amyloliquefaciens subsp.plantarum.The protease activity of the mutant strain which through the twice UV mutation was 147.1% of the original strain B15.The amylase production was positively correlated with protease production in this study.

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What this paper is about

Bacillus sp.B15which produces protease,amylase and antagonistic substance was identified by 16S rDNA sequence analysis.The homology of 16S rDNA sequence of the strain B15was 99% compared with Bacillus amyloliquefaciens subsp.plantarum AS43.3,and the strain B15was identified as Bacillus amyloliquefaciens subsp.plantarum.The protease activity of the mutant strain which through the twice UV mutation was 147.1% of the original strain B15.The amylase production was positively correlated with protease production in this study.

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Available abstract

Bacillus sp.B15which produces protease,amylase and antagonistic substance was identified by 16S rDNA sequence analysis.The homology of 16S rDNA sequence of the strain B15was 99% compared with Bacillus amyloliquefaciens subsp.plantarum AS43.3,and the strain B15was identified as Bacillus amyloliquefaciens subsp.plantarum.The protease activity of the mutant strain which through the twice UV mutation was 147.1% of the original strain B15.The amylase production was positively correlated with protease production in this study.

Key concepts: Bacillus amyloliquefaciens, Protease, Strain (injury), Biology, 16S ribosomal RNA, Mutant, Bacillus (shape), Amylase

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