Cloning and Sequence Analysis of Glycerol-3-phosphate Acyltransferase Genes in Peanut

Fatty acid desaturases are responsible for the insertion of double bonds into pre-formed fatty acid chains in reactions that require oxygen and reducing equivalents.Through construction of a full-length cDNA library of peanut(Arachis hypogaea)seed,expressed sequence tag sequencing and rapid amplification of cDNA ends,two fatty acid desaturase genes,designated AhFAB2-2 and AhFAB2-3 were isolated from peanut.The sequence of AhFAB2-2 and AhFAB2-3cDNA were 1387bp and 1452bp,and ORF were 1158bp and 1188bp,respectively.The molecular weight of AhFAB2-2 and AhFAB2-3 were predicted to be 43.7kD and 44.9kD,and isoelectric points 5.69 and 6.37,respectively.The deduced amino acid had a high identity with the FAB2 proteins reported from other species.Amino acid similarities of AhFAB2-2 and AhFAB2-3 between peanut and Arabidopsis thaliana were 60.8% and 57.2%,respectively;and amino acid similarities of AhFAB2-2and AhFAB2-3 protein between peanut and Glycine max were 62.3% and 59.3%,respectively.The sequences of the two genes were submitted to GenBank and their accession number were KF358459 and KF358460,respectively.