Histological Structure on Midbrain of Bufo gargarizans

HE staining and Holmes silver staining methods were used to reveal the histological structure of the midbrain in Bufo gargarizans.In dorsal midbrain,the optic lobe can be divided into tectum and tegmentum.From lateral to medical,tectum includes eight layers: stratum zonale,outer gray layer,superficial white layer,middle gray layer,middle white layer,deep gray layer,deep white layer and central gray substance.The anterior of tegmentum organization is the same as tectum,but the layers in the posterior of tegmentum are not obvious.Ventral midbrain includes the tegmentum and the cerebral peduncle.The color of cerebral peduncle becomes darker from lateral to medical by HE staining and Holmes silver staining,there are a large number of longitudinal nerve fibers in cerebral peduncle.It's the transverse nerve fibers in the boundary department of two crus cerebris.The cells in lateral tegmentum are not stratified and gathered into nucleus.In medialis tegmetum,cells and fibers are sub-eight layers concentric rings as the center of the midbrain aqueduct.By comparing with the difference of toads' midbrain dorsal and ventral,we can understand different functions of the dorsal and ventral.The characters of midbrain in Bufo gargarizans were compared with other vertebrate animals.