Improvement of potato dextrose agar medium preparation

To get a potato dextrose agar medium which is easy to make,economical and has higher yeast counting ratio,medium preparation method was improved.The main improvement was directly using potato mashing without filtration.Validation of improved media was evaluated by screening of potato concentration,yeast capability testing experiments and yeast counting experiments.The results indicated that the optimal potato concentration was 100g/L,and it had no significant influence on yeast capability compared with the traditional potato dextrose agar medium.The improved potato dextrose agar medium had a 67.2% higher yeast counting ratio than that of the traditional,and the t-test showed the two ratios have significant difference.With the improved method,the new potato dextrose agar saved material,had better capability in counting yeast and can be made easily.