Enzymatic synthesis of structured lipids in a solvent-free system

Lipase-catalyzed acidolysis of rapeseed oil with caprylic acid was performed to produce low-calorie structured lipids in a solvent-free system.Six commercial lipases were screened for their ability to catalyze acidolysis and the results indicated that the immobilized Lipozyme RM IM had more catalysis ability and Sn-1,3-specificity.Acidolysis parameters,such as time,reaction temperature,substrate molar ratio,enzyme load,water content were investigated.Under these conditions of 10% of the total weight of substrates for immobilized lipase,molar ratio of rapeseed oil to caprylic acid 1∶4 and reaction 15 h at 50℃,the incorporation of caprylic acid into rapeseed oil reached 40%.