Enzymatically Modified of Soybean Oil to Produce Structured Lipids

The lipase-catalyzed acidolysis of soybean oil with caprylic acid to produce structured lipids was investigated. Of the three immobilized lipases tested, lipozyme RM IM from Rhizomucor miehei resulted in the highest caprylic acid incorporation into the soybean oil. This enzyme was further studied for the effects of enzyme load, substrate ratio, added water content, reaction temperature and time, respectively. The results showed that for 500 mg soybean oil, the optimal reaction conditions are as follows: reaction temperature 40℃, immobilized lipase 472 mg, mole ratio of substrates 6:1(caprylic acid/soybean oil), added water content 9.7μl, and reacting time 24 h. The incorporation of caprylic acid reaches 43%, and the fatty acid contained in the structured lipids is the best.